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Hi! I'm just a little confused with respect to finding the pI of an amino acid with multiple charged groups. Which is correct? 1) The pI = the average of all the pKa values of each group (pka1+pka2+pka3/3) or 2) The pI = the average of the pKa values on either side of the zwitterion? If you could provide an example with the explanation I would REALLY appreciate it! Thanks!